Journal: bioRxiv

Article Title: Secretory leukocyte protease inhibitor influences periarticular joint inflammation in B. burgdorferi -infected mice

doi: 10.1101/2024.11.24.625079

Figure Lengend Snippet: (A) Sandwich ELISA results show the interaction of B. burgdorferi whole cell lysates with human SLPI. ELISA plates were coated with B. burgdorferi whole cell lysates and probed with increasing amount of human SLPI (blue) or human Fc proteins (black) as the negative control. The values plotted represent the mean ± SEM of duplicates from two experiments. P -value is displayed in the graph and determined using the non-parametric Mann-Whitney test. (B and C) Flow cytometry histograms show binding of human (B) and murine (C) SLPI to B. burgdorferi cultured at 33°C (solid line) and 37°C (dash line). B. burgdorferi was cultured to a density of 10 6 /ml. The same volume of cultures was incubated at 33°C or 37°C for 24 hrs before adding 10 nM (green) or 1 μM (red) human or murine SLPI. The binding was detected with goat anti human or murine SLPI and donkey anti goat AF647 or AF488. B. burgdorferi alone (grey) and antibody control (blue) were used as negative controls. (D) Immuno-fluorescent microscopy was used to directly observe the binding of B. burgdorferi with human and murine SLPI. Merged and single-color images are shown. Scale bar: 10 μm.

Article Snippet: The cells were pelleted, rinsed, resuspended, and incubated with 10 nM and 1 µM human SLPI (R&D Systems, #1274-PI-100) and murine SLPI (produced in lab as described above) at 4°C for 2 h. The binding was detected with goat anti human or murine SLPI (R&D Systems, #AF1274 and AF1735) and Alexa Fluor 488 or Alexa Fluor 647 donkey anti goat IgG (H+L) (Invitrogen, #A32814, A-21447).

Techniques: Sandwich ELISA, Enzyme-linked Immunosorbent Assay, Negative Control, MANN-WHITNEY, Flow Cytometry, Binding Assay, Cell Culture, Incubation, Control, Microscopy

Journal: iScience

Article Title: Identification of proteotoxic and proteoprotective bacteria that non-specifically affect proteins associated with neurodegenerative diseases

doi: 10.1016/j.isci.2024.110828

Figure Lengend Snippet:

Article Snippet: Fusobacterium gonidiaformans , BEI Resources , HM-1274.

Techniques: Virus, Recombinant, Membrane, Western Blot, Software